生物資訊中心 (Bioinformatics Center)

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註解基因之範例說明

Annotation procedures:

        Automatic analysis:

                Homology search: Blastn (UniGene, Repbase), Blastx (GenPept)

                Exon prediction: Genscan, FgeneH, Grail

                Sequence feature prediction: Promoter, ORF, Start/Stop codons, Splice sites

        Manunal inspection:

                Gene annotation: Gene finding & classification

                Splice site adjustment

        Experimental verification:

                RT-PCR

Annotator Browser:

Classification of Annotated Genes:

Known genes (Well-known cDNA defined):

Highly homologous or identical to known human proteins or genes.

e.g., NFKB1

 e.g., Hexokinase III

Related genes (GenPept hit, Orthologs or Paralogs):

Homologous or similar to the existed known proteins or genes.

e.g., UNC5H1 homolog

Predicted genes (EST-defined):

Highly homologous to EST sequences only.

e.g., Eukaryotic translation elongation factor 1 alpha subunit

   (Hs. 29835, Hs. 181165)

Pseudogenes:

Homologous to existed gene but with disrupted ORF.

e.g., Keratin 8 pseudogene

Related genes (GenPept hit, Orthologs or Paralogs):

Homologous or similar to the existed known proteins or genes.

e.g., Class IV alcohol dehydrogenase homolog