Sequence-Based Linkage
Analysis
Sep. 20, 2004
Jay-chang Chen
Abstract
With the rapid development of DNA sequencing, we could
apply that for other usages. In this paper, the authors propose resequcing a
lot of short contiguous segments of genomic DNA（sequencing blocks【SBs】） to simultaneous
discover markers and get genotype polymorphisms in each linkage studies. In
spite of the frequency of all sequence polymorphisms in the SBs, they would be
used as genetic markers.
The Author use computer simulations to test the method.
They created pedigree data with population theory and observed human sequence
diversity first. After the simulation, they found that SBs of 500-1000 bp,
spaced at intervals of 1-2 Mb across the genome, provide more information than
traditional approaches.
For validation of the result, they apply the approach for
chromosome
From: American
Journal of Human Genetics (2004) 75:647-653
References
*1.
Itay Furman, Mark J. Rieder, Suzanne da Ponte,
Dana P. Carrington, Deborah A. Nickerson,
Leonid Kruglyak, and Kyriacos Markianos. (2004) Sequenced-Based
Linkage Analysis. Am. J. Hum. Genet. 75: 647-653.
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