The Small RNA Chaperone Hfq and Multiple Small RNAs Control Quorum Sensing in Vibrio harveyi and Vibrio cholerae

September 27, 2004

章人勻

Abstract:

Continue with former study, author finds a new component, Hfq, of the V. harveyi and V. cholerae Quorum-sensing circuits. It mediates interactions between small, regulatory RNAs and specific mRNA, and these interactions cause destabilization of the mRNA of the Quorum-sensing master regulators LuxR ( V. Harveyi ) and HapR ( V. cholerae ). Then identified four putative sRNAs in V. cholerae by bioinformatic tools and find out simultaneous deletion of all four sRNAs is required to stabilized hapR mRNA. Last author propose that Hfq, together with these sRNAs, creates an ultra sensitive regulatory switch to control the Quorum-sensing mode.

From Cell, Vol. 118, 69-82, July 9, 2004

http://www.cell.com/content/article/abstract?uid=PIIS0092867404005732

reference:

1. Lilley, B.N., and Bassler, B.L. (2000). Regulation of quorum sensing in Vibrio harveyi by LuxO and sigma-54. Mol. Microbiol. 36, 940–954

2. Miller, M.B., and Bassler, B.L. (2001). Quorum sensing in bacteria. Annu. Rev. Microbiol. 55, 165–199

3. http://www.molbio.princeton.edu/research_facultymember.php?id=5