Microarray analysis of microRNA expression in the developing mammalian brain

Speaker : ªLÁ¾¼w

ABSTRACT

microRNAs (miRNAs) are small, functional, noncoding RNAs.

miRNAs are transcribed as long primary transcripts (primary precursors) that are processed to the ~75 nt precursors (pre-miRNAs) by the nuclear enzyme Drosha.

The ~22 nt mature miRNA is processed from the pre-miRNA by the RNase III Dicer.

The goal of this report was to develop a high-throughput method to mammalian brain.

The result is comparison of microarray data with representative developmental northern blots of microRNAs.

From : http://genomebiology.com/2004/5/9/R68

Reference

1. Krichevsky AM, King KS, Donahue CP, Khrapko K, Kosik KS: A microRNA array reveals extensive regulation of microRNAs during brain development. RNA 2003, 9:1274-1281.

2. Griffiths-Jones S: The miRNA Registry. Nucleic Acids Res 2004, 32

Database issue:D109-D111.