Cell Cycle Regulated Transport Controlled by Alterations in the Nuclear Pore Complex

April 27 2004

邱裕倫

Abstract

Makhneych et. al., interested in the regulatory mechanisms of the nuclear transport of macromolecules that control various cellular events including movement through the stage of cell cycle. In yeast cells, the nuclear envelope remains intact throughout the cell cycle and the transport regulatory mechanisms must be function during mitosis. The authors have uncovered a mechanism for regulating transport that is controlled by M phase specific molecular rearrangements in the Nuclear Pore Complex(NPC).These changes allow a transport inhibitory nucleoporin, Nup53, to bind the karyopherin Kap121p specifically during mitosis, slowing its movement through the (NPC) and inducing cargo release. The authors proposed the fluctuations in Kap121p transport mediated by the NPC contribute to controlling the subcellular distribution of molecules that directly progression through mitosis.

Source: Taras Makhnevych, C. Patrick Lusk, Andrea M. Anderson, John D. Aitchson, and Richard W. Wozniak. 2003. Cell. 115：813-823.

Reference

1. Michael P. Rout and John D. Aitchson. 2001. The Nuclear Pore Complex as a Transport Machine. The JOURNAL OF BIOLOGICAL CHEMISTRY. 276：16593-16596.

2. Karsten Weis. 2003. Regulating Access to the Genome: Nucleocytoplasmic Transport throughout the Cell Cycle. Cell.112：441-451.