g39119013魏正岷05/11

Abstract:

The authors integrated expression database mining & subtractive hybridization to find "Endothelial-Enriched" candidate genes. In order to validate these candidates, they isolated RNA from 9 different vascular or non-vascular cell cultures, to contract Microarray experiment. After analysis Microarray data, 64 pan-endothelial markers that were differentially expressed. In addition, differences in gene expression profiles among endothelial cells from different vascular beds were identified. Validation of these findings was performed by RNA blot expression studies, and a number of the novel genes were shown to be expressed under angiogenic conditions in the developing mouse embryo.

Paper abstract: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12644598

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