Mutants

Vocabulary of mutation

• Size
  • Point mutation
  • Deletion / Insertion
• Effect on function
  • Missense: caused by point mutation
  • Nonsense: caused by point mutation
  • Frameshift: caused by deletion or insertion
  • Silent mutation
• Effection on another gene
  • gain of function mutation
  • suppressor mutation: ochre, opal
• Effect on survival
  • lethal mutation
  • neutral mutation
  • leaky mutation
• Type
  • normal (fixed ?)
  • conditional: temperature sensitive, cold sensitive, host range, ---etc.

Artificial

• Random: chemical derived, PCR-derived
• Site-specific
• Linker scanning mutants
• Cassette mutagenesis
• 　

Strategies and assumption