Rate-limiting step

反應速率決定步驟是連續反應中最慢的一步,即瓶頸反應(bottle neck).

If you want to increase the rate of a pathway, you should regulate the rate of the rate-limiting step (RLS). Otherwise, there will be no effect.

Rate-limiting step is different from flux-generating reaction by definition. Nevertheless, sometimes RLS is also the flux-generating reaction.