序列資訊之應用

1997/8/20


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目錄

序列資訊之應用

Approaches for Cloning A Disease-causing Gene

How to Clone A Disease-causing Gene

Positional Cloning Moves from Perditional to Traditional?Francis S. Collins, Nature Genetics, 1995

The Major Aims of Human Genome Project

Anticipated Approaches in Future Molecular Medicine

Two Different Approaches in Human Genome Project

Two Major Problems in Human Genome Project

How to Assemble Sequences from Human Genome Project?

Genome-wide STS-based Human Maps

How Is Expressed Sequence Tags(EST) Generated?

Application of ESTs

The Turning Point in Genome Research?Mark S. Boguski?Trends in Biochemical Sciences 20:295-6

Statics of dbEST

How Can I Use EST Information as Research Tools?

PowerPoint 簡報

cDNA Clones Containing ESTs Are Available

How to Order cDNA Clones Containing ESTs?

A Real Example of Applying?EST Information to? Biological Research

PowerPoint 簡報

PowerPoint 簡報

Remote Search of dbEST by Using BLAST

PowerPoint 簡報

Alignment of EST W31907, W39270, and se16 with Mouse eIF4G-related Gene

Identity between Different eIF4G Related Genes

Analyze ESTs Manually

96 ESTs Were Found by BLAST Searching dbEST with Mouse eIF4G-related Gene

Batch Entrez Retrieve ESTs from dbEST

Gis/Accession File Format

How to Merge ESTs into Contigs?

Use GelMerge to Merge ESTs into Contigs

Contigs Generated from Sequences Processed by GelMerge

Sequence Assembly of Mouse eIF4G-related EST's

PowerPoint 簡報

PowerPoint 簡報

296 ESTs Were Found by BLAST Searching dbEST with Mouse eIF4G-related Gene

Alignment of mouse eIF4G-related ESTs with p97 mRNA

Conclusion

Some Extra Sequences in Contigs Cannot Align to p97

Sources of Extra Sequences in Contigs

How to Predict Splicing Junctions by Sequence Alignment of Contigs

Prediction of Splicing Junctions in p97 mRNA

PowerPoint 簡報

Conclusion

Summary

作者:黃彥華

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