Putative ALternative Splicing Database
[Introduction] [Rationale] [History] [Statistics] [User's Guide] [Chinese Version] [Mirror Sites] [Supplementary Material]
Putative ALternative Splicing Database (PALS db) is a database designed to provide biologists a wide coverage of putative alternative splicing (AS) sites to all known human genes. We used the unique sequences of UniGene clusters as reference sequences to construct PALS db.
To collect the human and mouse EST sequences that are absent in the UniGene database, the reference sequence was used as a query sequence for blast analysis against dbEST. The splicing sites can then be discovered by comparing reference sequence with other sequences in the same UniGene cluster or with related EST sequences from dbEST.
The work of us differs from other available databases in that we tried to cover all known human genes and all available sources of sequences possibly containing AS information. Though genomic sequences are the absolute reference sequences to find AS, half of human genome draft is still unordered and fragmented, which makes alignment more complicated.
For biologists, the more information in the database means the more chances they can find significant phenomena. The more updated information means the earlier they can get the first hand information. The more information links to AS information means the easier they can propose a hypothesis. Thus, a database that can fulfill these needs for biologists will be useful and PALS db is designed for this purpose.
After release 2, the mouse genes were also incorporated into PALS db. See History, Statistics for details.
Current release information can be found at the pages: Release note and , History.